ABSTRACT
Objective: To obtain the bFGF mimic peptide binding to FGFR via phage display, and to provide the base for developing peptide agonist of bFGF. Methods: Using Balb/c 3T3 cells as the target cells and COS-7 cells as the subtractive panning, the phage display heptapeptide library was biopanned for 4 rounds to obtain the single phage clones. The affinity and the specificity of the clones were assessed by ELISA. DNA sequencing was applied to further analyze the positive clones. Results: Twelve positive clones were selected from the enriched phages. A group of hydrophobic peptides containing a conserved motif, PR, was identified. Conclusion: Two bFGF mimic heptapeptides binding to FGFR were selected, which may be used as the candidates for bFGF agonist.
ABSTRACT
The aim of this study was to investigate the initial time of platelet inhibiting effect of aspirin (ASA) and the effects of different doses on equilibrium of prostacyclin (PGI(2))-thromboxane B(2) (TXB(2)). The effects of 100 mg and 300 mg ASA on Platelet count, platelet aggregation rate, TXB(2) and PGI(2) were investigated using cross-compare way for 40 aspirin ingestion patients. The results showed that the platelet counts decreased to 33% after 30 minutes of single-dose ASA ingestion of 100 mg and to 25.6% after 60 minutes. TXB(2) and PGI(2) also decreased meanwhile. The platelet counts decreased to 39.5%, 35.5% and 26.6%, respectively with dose of 300 mg on day 1, 2 and 3. The platelet counts decreased to 38.1% and 39.5%, respectively, after 120 minutes with 100 and 300 mg ASA ingestion, without significent difference. In conclusion ASA began to inhibit platelet function after 30 minutes of ingestion, and gave the strongest inhibition after 60 minutes. Continuous ASA ingestion accumulates the inhibitory effect. The single-dose ASA ingestion of 100 and 300 mg have nearly the same inhibitory effects.